The effect of high intensity interval training on gene expression of inflammatory biomarkers in fast- and slow muscles of rats

Abstract

Background and aims: Lipocalin-2 (LCN-2) with Interleukin1-β (IL1-β) are inflammatory proteins that induced the inflammatory reactions due to insufficient of physical activity. The purpose of the current study was to determine the effect of high intensity interval training on Lipocalin-2 and Interleukin1-β gene expressions in fast- and slow muscles of rats. Methods: In the current study 20 Wistar rats were divided into HIIT and control groups. HIIT protocol was performed 60 min in each session for four sessions in each week. After warm-up, HIIT group was carried out 15 × 4 min bouts of HIIT with 85 to 90% of VO2max that continued with three min recovery (between each bout of HIIT) with 70% of VO2max. LCN-2 and IL1-β and their gene translations were evaluated by commercial kits and Real-Time PCR method, respectively.    Results: Findings shown that VO2max was significantly increased and serum levels of LCN-2 and IL1-β were significantly decreased (p<0.05). Also, LCN-2 and IL1-β gene translations in both fast- and slow muscles were significantly decreased (p<0.05).   Conclusion: It seems that, current HIIT protocol has capability to significantly decrease both muscular and serum levels of inflammatory biomarkers such as LCN-2 and IL1-β in male rats. Particularly, results of current study shown that LCN-2 and IL1-β decreases are greater in EDL muscles.            

Keywords

• Gene expression
• Inflammatory biomarkers
• Lipocalin-2