Autologous ADSCs with exogenous NPY promotes fracture healing in ovariectomized rats
Hong Liu,
Jiaoyang Wu,
Huixuan Wu,
Ting Wang,
Houde Zhou,
Min Liu
Affiliations
Hong Liu
Department of Nutrition, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China
Jiaoyang Wu
Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China
Huixuan Wu
National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory for Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second XiangYa Hospital of Central South University, Changsha, Hunan 410011, PR China
Ting Wang
National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory for Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second XiangYa Hospital of Central South University, Changsha, Hunan 410011, PR China
Houde Zhou
National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory for Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second XiangYa Hospital of Central South University, Changsha, Hunan 410011, PR China; Corresponding author. National Clinical Research Center for Metabolic Diseases, the Second XiangYa Hospital of Central South University, 139 Renmin Middle Road, Changsha, Hunan 410011, PR China.
Min Liu
Department of Nutrition, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China; Corresponding author. Department of Nutrition, the Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, Hunan 410013, PR China.
Objective: To evaluate the role of autologous adipose-derived stem cells (ADSCs) with exogenous neuropeptide Y (NPY) on osteoporotic fracture healing. Methods: We established a fracture healing model in ovariectomized rats of osteoporosis. Autologous ADSCs, isolated from rats' subcutaneous adipose tissue, with exogenous NPY were transplanted into the fracture site four times a week. The fracture healing was observiked with X-ray diagnostic techniques, and the rats’ tibias were taken out for section-staining and micro-CT scanning 3 and 6 weeks after fracture. Next, we explored the underlying mechanism by which the transplantation contributed to fracture healing. Results: Co-culture and co-transplantation of ADSCs with NPY could stimulate the osteoporotic fracture healing. NPY may promote the osteogenic differentiation of ADSCs through Y1 receptor. Conclusion: This result provides a platform for the development of novel therapies for bone regeneration with endogenous ADSCs.
