Phylogenetic analysis and designing new primers for molecular identification of Drosophila suzukii

Abstract

The family Drosophilidae includes over 3750 species worldwide and over 2000 of these are species of Drosophila. Spotted wing drosophila (SWD), Drosophila suzukii is one of the most dangerous species in this family. The insects live on undamaged ripening fruits, using its peculiar serrated ovipositor to break the skin of fresh ripening fruits and lay eggs in it. Drosophila species are very difficult and practically impossible to detect at larval stages. The present investigation was conducted at the All-Russian Plant Quarantine Center and Agrarian and Technological Institute of RUDN University, Moscow, Russia in 20182020. The aim of this study was to investigate the method of accurate and rapid identification of D. suzukii, and to design specific primer pairs for pest identification by Real-Time PCR method. The real-time quantitative PCR is a fast, sensitive, repeatable and accurate method for quantifying gene transcript levels. In this study, we designed specific primers (4.Dsuz.FRP) for Real-Time PCR to identify D. suzukii from other relative species. Although D. suzukii is absent in the Russian Federation and has not been reported so far, the project could be a precautionary measure.

Keywords

• identification
• original sequences
• phylogenetic tree
• drosophila suzukii
• pcr
• plant quarantine