MSPoisDM: A Novel Peptide Identification Algorithm Optimized for Tandem Mass Spectra

Abstract

Tandem mass spectrometry (MS/MS) plays an extremely important role in proteomics research. Thousands of spectra can be generated in modern experiments, how to interpret the LC-MS/MS is a challenging problem in tandem mass spectra analysis. Our peptide identification algorithm, MSPoisDM, is integrated the intensity information which produced by target-decoy statistics, although intensity information often undervalued. Furthermore, in order to combine the intensity information for better, we propose a novel concept scoring model which based on Poisson distribution. Compared with commonly used commercial software Mascot and Sequest at 1% FDR, the results show MSPoisDM is robust and versatile for various datasets which obtained from different instruments. We expect our algorithm MSPoisDM will be broadly applied in the proteomics studies.

Keywords

• index-terms tandem mass spectrum
• protein identification algorithms
• weight of pair amino acid fracture