The in vitro effects of CoCl2 as ethylene synthesis inhibitor on PI based protein pattern of potato plant (Solanum tuberosum L.)

Abstract

The effect of CoCl2 as an ethylene synthesis inhibitor on changes of protein pattern was investigated in potato (Solanum tuberosum L.) plants cultivar White Desiree. In vitro grown plants were subjected to MS medium containing 0, 5, 10, 15, 20, 30, 40 and 60 mg/l CoCl2 for 4 weeks. Different concentrations of CoCl2 showed significant effect on the total soluble proteins. Among different concentrations of CoCl2, using 20 mg/l CoCl2 was the best concentration to inhibit ethylene formation and induce potato plant growth. Application of CoCl2 in the culture medium changed the total protein as well as SDS-PAGE and Iso-electric Point Electrophoresis (PI) patterns. Protein pattern in potato tuber did not show any detectable changes.

Keywords

• potato
• cocl2
• ethylene
• protein pattern