PLoS ONE (Jan 2014)

Plasma microRNAs as potential noninvasive biomarkers for in-stent restenosis.

  • Meijiao He,
  • Yongtai Gong,
  • Jing Shi,
  • Zhenwei Pan,
  • Hui Zou,
  • Danghui Sun,
  • Xin Tu,
  • Xiangyang Tan,
  • Jianqiang Li,
  • Weimin Li,
  • Bin Liu,
  • Jingyi Xue,
  • Li Sheng,
  • Chunhong Xiu,
  • Ning Yang,
  • Hongjie Xue,
  • Xue Ding,
  • Chengyuan Yu,
  • Yue Li

DOI
https://doi.org/10.1371/journal.pone.0112043
Journal volume & issue
Vol. 9, no. 11
p. e112043

Abstract

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OBJECTIVE: To investigate whether microRNAs (miRs) can serve as novel biomarkers for in-stent restenosis (ISR). METHODS: This retrospective, observational single-centre study was conducted at the cardiovascular department of a tertiary hospital centre in the north of China. Follow-up coronary angiography at 6 to 12 months was performed in 181 consecutive patients implanted with drug-eluting stents. Fifty-two healthy volunteers served as the control group. The plasma miRs levels were analyzed by quantitative real-time PCR. Receiver-operating characteristic curve (ROC) analysis was performed to investigate the characters of these miRs as potential biomarkers of ISR. RESULTS: MiR-21 levels in ISR patients were significantly higher than those in non-ISR patients and healthy controls (P<0.05), while miR-100 (P<0.05), miR-143 (P<0.001) and miR-145 (P<0.0001) levels were significantly decreased in ISR patients. Further analysis showed that miR-21 levels were remarkably increased (P = 0.045), while miR-100 (P = 0.041), miR-143 (P = 0.029) and miR-145 (P<0.01) levels were dramatically decreased in patients with diffuse ISR compared to those with focal ISR. ROC analysis demonstrated that the area under curve of miR-145, miR-143, miR-100 and miR-21 were 0.880 (95% confidence interval; CI = 0.791-0.987, P<0.001), 0.818 (95% confidence interval; CI = 0.755-0.963, P<0.001), 0.608 (95% confidence interval; CI = 0.372-0.757, P<0.05) and 0.568 (95% confidence interval; CI = 0.372-0.757, P<0.05), with specificity of 83.1%, 80.1%, 68.9% and 68.6%, and sensitivity of 88.7%, 82.1%, 60.2% and 50.1%, respectively. CONCLUSIONS: Circulating miR-143 and miR-145 levels are associated with the occurrence of ISR and can serve as novel noninvasive biomarkers for ISR.