A novel mistranslating tRNA model in <i>Drosophila melanogaster</i> has diverse, sexually dimorphic effects

Joshua R Isaacson; Matthew D Berg; Brendan Charles; Jessica Jagiello; Judit Villén; Christopher J Brandl; Amanda J Moehring

doi:10.1093/g3journal/jkac035

G3: Genes, Genomes, Genetics (Feb 2022)

A novel mistranslating tRNA model in <i>Drosophila melanogaster</i> has diverse, sexually dimorphic effects

Joshua R Isaacson,
Matthew D Berg,
Brendan Charles,
Jessica Jagiello,
Judit Villén,
Christopher J Brandl,
Amanda J Moehring

Affiliations

Joshua R Isaacson: ORCiD; Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada
Matthew D Berg: ORCiD; Department of Biochemistry, The University of Western Ontario, London, ON N6A 5B7, Canada
Brendan Charles: Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada
Jessica Jagiello: Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada
Judit Villén: ORCiD; Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
Christopher J Brandl: ORCiD; Department of Biochemistry, The University of Western Ontario, London, ON N6A 5B7, Canada
Amanda J Moehring: Department of Biology, The University of Western Ontario, London, ON N6A 5B7, Canada

DOI: https://doi.org/10.1093/g3journal/jkac035
Journal volume & issue: Vol. 12, no. 5

Abstract

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AbstractTransfer RNAs (tRNAs) are the adaptor molecules required for reading the genetic code and producing proteins. Transfer RNA variants can lead to genome-wide mistranslation, the misincorporation of amino acids not specified by the standard genetic code into nascent proteins. While genome sequencing has identified putative mistranslating transfer RNA variants in human populations, little is known regarding how mistranslation affects multicellular organisms. Here, we create a multicellular model of mistranslation by integrating a serine transfer RNA variant that mistranslates serine for proline ( tRNA UGG,G26A SerDrosophila melanogaster tRNA UGG,G26A Ser tRNA UGG,G26A Ser tRNA UGG,G26A Ser

Published in G3: Genes, Genomes, Genetics

ISSN: 2160-1836 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://academic.oup.com/g3journal

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