Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production

Chen-Guang Liu; Kai Li; Ke-Yi Li; Chularat Sakdaronnarong; Muhammad Aamer Mehmood; Muhammad Aamer Mehmood; Xin-Qing Zhao; Feng-Wu Bai

doi:10.3389/fbioe.2020.00615

Frontiers in Bioengineering and Biotechnology (Jun 2020)

Intracellular Redox Perturbation in Saccharomyces cerevisiae Improved Furfural Tolerance and Enhanced Cellulosic Bioethanol Production

Chen-Guang Liu,
Kai Li,
Ke-Yi Li,
Chularat Sakdaronnarong,
Muhammad Aamer Mehmood,
Muhammad Aamer Mehmood,
Xin-Qing Zhao,
Feng-Wu Bai

Affiliations

Chen-Guang Liu: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Kai Li: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Ke-Yi Li: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Chularat Sakdaronnarong: Department of Chemical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand
Muhammad Aamer Mehmood: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Muhammad Aamer Mehmood: Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad, Pakistan
Xin-Qing Zhao: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
Feng-Wu Bai: State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

DOI: https://doi.org/10.3389/fbioe.2020.00615
Journal volume & issue: Vol. 8

Abstract

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Furfural is a major toxic byproduct found in the hydrolysate of lignocellulosic biomass, which adversely interferes with the growth and ethanol fermentation of Saccharomyces cerevisiae. The current study was focused on the impact of cofactor availability derived intracellular redox perturbation on furfural tolerance. Here, three strategies were employed in cofactor conversion in S. cerevisiae: (1) heterologous expression of NADH dehydrogenase (NDH) from E. coli which catalyzed the NADH to NAD+ and increased the cellular sensitivity to furfural, (2) overexpression of GLR1, OYE2, ZWF1, and IDP1 genes responsible for the interconversion of NADPH and NADP+, which enhanced the furfural tolerance, (3) expression of NAD(P)+ transhydrogenase (PNTB) and NAD+ kinase (POS5) which showed a little impact on furfural tolerance. Besides, a substantial redistribution of metabolic fluxes was also observed with the expression of cofactor-related genes. These results indicated that NADPH-based intracellular redox perturbation plays a key role in furfural tolerance, which suggested single-gene manipulation as an effective strategy for enhancing tolerance and subsequently achieving higher ethanol titer using lignocellulosic hydrolysate.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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