Frontiers in Immunology (Mar 2023)
Convalescent human IgG, but not IgM, from COVID-19 survivors confers dose-dependent protection against SARS-CoV-2 replication and disease in hamsters
- Hannah A. D. King,
- Hannah A. D. King,
- Hannah A. D. King,
- Vincent Dussupt,
- Vincent Dussupt,
- Vincent Dussupt,
- Letzibeth Mendez-Rivera,
- Letzibeth Mendez-Rivera,
- Letzibeth Mendez-Rivera,
- Bonnie M. Slike,
- Bonnie M. Slike,
- Bonnie M. Slike,
- Ursula Tran,
- Ursula Tran,
- Ursula Tran,
- Nathan D. Jackson,
- Nathan D. Jackson,
- Nathan D. Jackson,
- Erica Barkei,
- Michelle Zemil,
- Michelle Zemil,
- Michelle Zemil,
- Emily Tourtellott-Fogt,
- Emily Tourtellott-Fogt,
- Emily Tourtellott-Fogt,
- Caitlin H. Kuklis,
- Sandrine Soman,
- Aslaa Ahmed,
- Maciel Porto,
- Christopher Kitajewski,
- Brittany Spence,
- Dalia Benetiene,
- Lindsay Wieczorek,
- Lindsay Wieczorek,
- Lindsay Wieczorek,
- Swagata Kar,
- Gregory Gromowski,
- Victoria R. Polonis,
- Shelly J. Krebs,
- Shelly J. Krebs,
- Shelly J. Krebs,
- Kayvon Modjarrad,
- Diane L. Bolton,
- Diane L. Bolton,
- Diane L. Bolton
Affiliations
- Hannah A. D. King
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Hannah A. D. King
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Hannah A. D. King
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Vincent Dussupt
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Vincent Dussupt
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Vincent Dussupt
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Letzibeth Mendez-Rivera
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Letzibeth Mendez-Rivera
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Letzibeth Mendez-Rivera
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Bonnie M. Slike
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Bonnie M. Slike
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Bonnie M. Slike
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Ursula Tran
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Ursula Tran
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Ursula Tran
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Nathan D. Jackson
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Nathan D. Jackson
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Nathan D. Jackson
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Erica Barkei
- Veterinary Pathology Branch, WRAIR, Silver Spring, MD, United States
- Michelle Zemil
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Michelle Zemil
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Michelle Zemil
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Emily Tourtellott-Fogt
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Emily Tourtellott-Fogt
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Emily Tourtellott-Fogt
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Caitlin H. Kuklis
- Viral Diseases Branch, WRAIR, Silver Spring, MD, United States
- Sandrine Soman
- Viral Diseases Branch, WRAIR, Silver Spring, MD, United States
- Aslaa Ahmed
- Viral Diseases Branch, WRAIR, Silver Spring, MD, United States
- Maciel Porto
- BIOQUAL, Inc., Rockville, MD, United States
- Christopher Kitajewski
- BIOQUAL, Inc., Rockville, MD, United States
- Brittany Spence
- BIOQUAL, Inc., Rockville, MD, United States
- Dalia Benetiene
- BIOQUAL, Inc., Rockville, MD, United States
- Lindsay Wieczorek
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Lindsay Wieczorek
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Lindsay Wieczorek
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Swagata Kar
- BIOQUAL, Inc., Rockville, MD, United States
- Gregory Gromowski
- Viral Diseases Branch, WRAIR, Silver Spring, MD, United States
- Victoria R. Polonis
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Shelly J. Krebs
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Shelly J. Krebs
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Shelly J. Krebs
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- Kayvon Modjarrad
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Diane L. Bolton
- US Military HIV Research Program, Walter Reed Army Institute of Research (WRAIR), Silver Spring, MD, United States
- Diane L. Bolton
- Emerging Infectious Diseases Branch, WRAIR, Silver Spring, MD, United States
- Diane L. Bolton
- Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, United States
- DOI
- https://doi.org/10.3389/fimmu.2023.1138629
- Journal volume & issue
-
Vol. 14
Abstract
IntroductionAntibody therapeutic strategies have served an important role during the COVID-19 pandemic, even as their effectiveness has waned with the emergence of escape variants. Here we sought to determine the concentration of convalescent immunoglobulin required to protect against disease from SARS-CoV-2 in a Syrian golden hamster model.MethodsTotal IgG and IgM were isolated from plasma of SARS-CoV-2 convalescent donors. Dose titrations of IgG and IgM were infused into hamsters 1 day prior to challenge with SARS-CoV-2 Wuhan-1.ResultsThe IgM preparation was found to have ~25-fold greater neutralization potency than IgG. IgG infusion protected hamsters from disease in a dose-dependent manner, with detectable serum neutralizing titers correlating with protection. Despite a higher in vitro neutralizing potency, IgM failed to protect against disease when transferred into hamsters.DiscussionThis study adds to the growing body of literature that demonstrates neutralizing IgG antibodies are important for protection from SARS-CoV-2 disease, and confirms that polyclonal IgG in sera can be an effective preventative strategy if the neutralizing titers are sufficiently high. In the context of new variants, against which existing vaccines or monoclonal antibodies have reduced efficacy, sera from individuals who have recovered from infection with the emerging variant may potentially remain an efficacious tool.
Keywords
