Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

Jianpeng Ao; Xiaofeng Fang; Xianchong Miao; Jiwei Ling; Hyunchul Kang; Sungnam Park; Changfeng Wu; Minbiao Ji

doi:10.1038/s41467-021-23407-2

Nature Communications (May 2021)

Switchable stimulated Raman scattering microscopy with photochromic vibrational probes

Jianpeng Ao,
Xiaofeng Fang,
Xianchong Miao,
Jiwei Ling,
Hyunchul Kang,
Sungnam Park,
Changfeng Wu,
Minbiao Ji

Affiliations

Jianpeng Ao: State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University
Xiaofeng Fang: Department of Biomedical Engineering, Southern University of Science and Technology
Xianchong Miao: State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University
Jiwei Ling: State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University
Hyunchul Kang: Department of Chemistry and Research Institute for Natural Science, Korea University
Sungnam Park: Department of Chemistry and Research Institute for Natural Science, Korea University
Changfeng Wu: Department of Biomedical Engineering, Southern University of Science and Technology
Minbiao Ji: State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University

DOI: https://doi.org/10.1038/s41467-021-23407-2
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 8

Abstract

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Probes with reversible fluorescence are useful in super-resolution microscopy, but lack sufficient chemical specificity. Here, the authors engineer alkyne tagged diarylethene to realize photo-switchable stimulated Raman scattering probes with high chemical resolution, for applications in living cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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