Infection and Drug Resistance (Jul 2022)

Molecular Characterization of Klebsiella pneumoniae Isolated from Sputum in a Tertiary Hospital in Xinxiang, China

  • Hao Y,
  • Jiang Y,
  • Ishaq HM,
  • Liu W,
  • Zhao H,
  • Wang M,
  • Yang F

Journal volume & issue
Vol. Volume 15
pp. 3829 – 3839

Abstract

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Yuqi Hao,1 Yong’ang Jiang,1 Hafiz Muhammad Ishaq,2 Wenke Liu,1 Huajie Zhao,1 Mingyong Wang,3 Fan Yang1 1Xinxiang Key Laboratory of Pathogenic Biology, Department of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, People’s Republic of China; 2Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan; 3Xinxiang Key Laboratory of Immunoregulation and Molecular Diagnostics, School of Laboratory Medicine, Xinxiang Medical University,, Xinxiang, People’s Republic of ChinaCorrespondence: Fan Yang; Mingyong Wang, Email [email protected]; [email protected]: In clinical practice, Klebsiella pneumoniae (K. pneumoniae) is a common opportunistic pathogen responsible for nosocomial infection. This study aimed to analyze the trend of antimicrobial susceptibility and virulent characteristics of K. pneumoniae isolated from sputum. In clinics, data of the current study will help in the clinical treatment of K. pneumoniae infection.Results: The current research showed the resistance rates of the 20 K. pneumoniae isolates against 13 antibiotics ranged from 15.0% to 80.0%. The detection rate of extended spectrum β-lactamases (ESBLs) was up to 55%, while blaSHV was the most prevalent ESBLs genes. Four strains (25.0%) of K. pneumoniae presented hypermucoviscous phenotype (HMV). Moreover, 18 strains (90.0%) showed the stronger biofilm-forming ability. wzi, wabG, fimH, mrkD were the most prevalent virulence genes in current research. Ten strains were found capsule typing and the higher genetic diversity of colonizing K. pneumoniae in this region. K19 exhibited a strong positive correlation with imipenem resistance, while K1 showed strong correlations with magA. Furthermore, HMV phenotype showed significantly negative correlations with multidrug-resistant.Conclusion: In the hospital, the antibiotic resistance of K. pneumoniae (isolated from sputum samples) has a serious concern. Additionally, strains of K. pneumoniae show the higher genetic diversity.Keywords: Klebsiella pneumoniae, antimicrobial resistance, resistant genes, virulent genes, biofilm-forming

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