International Journal of Nanomedicine (Oct 2019)
Glucose oxidase complexed gold-graphene nanocomposite on a dielectric surface for glucose detection: a strategy for gestational diabetes mellitus
Abstract
Yueping Ge,1 Thangavel Lakshmipriya,2 Subash CB Gopinath,2,3 Periasamy Anbu,4 Yeng Chen,5 Firdaus Hariri,6 Lu Li7 1Department of Gynaecology and Obstetrics, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, Zhengzhou City, Henan Province 450003, People’s Republic of China; 2Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis 01000, Malaysia; 3School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau 02600, Perlis, Malaysia; 4Department of Biological Engineering, College of Engineering, Inha University, Incheon 402-751, Republic of Korea; 5Department of Oral & Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia; 6Department of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia; 7Department of Obstetrics, Jinan Central Hospital Affiliated to Shandong University, Jinan City, Shandong Province 250013, People’s Republic of ChinaCorrespondence: Lu LiDepartment of Obstetrics, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Rd, Lixia Qu, Jinan City, Shandong Province 250013, People’s Republic of ChinaEmail [email protected]: Gestational diabetes mellitus is a commonly occurring metabolic disorder during pregnancy, affecting >4% of pregnant women. It is generally defined as the intolerance of glucose with the onset or initial diagnosis during pregnancy. This illness affects the placenta and poses a threat to the baby as it affects the supply of proper oxygen and nutrients.Purpose: Due to the high percentage of affected pregnant women, it should be mandatory to evaluate glucose levels during pregnancy and there is a need for a continuous monitoring system.Methods: Herein, the investigators modified the interdigitated (di)electrodes (IDE) sensing surface to detect the glucose on covalently immobilized glucose oxidase (GOx) with the graphene. The characterization of graphene and gold nanoparticle (GNP) was performed by high-resolution microscopy.Results: Sensitivity was found to be 0.06 mg/mL and to enhance the detection, GOx was complexed with GNP. GNP-GOx was improved the sensitive detection twofold from 0.06 to 0.03 mg/mL, and it also displayed higher levels of current changes at all the concentrations of glucose that were tested. High-performance of the above IDE sensing system was attested by the specificity, reproducibility and higher sensitivity detections. Further, the linear regression analysis indicated the limit of detection to be between 0.02 and 0.03 mg/mL.Conclusion: This study demonstrated the potential strategy with nanocomposite for diagnosing gestational diabetes mellitus.Keywords: gestational diabetes, glucose oxidase, graphene, interdigitated electrode, gold nanoparticle
