Investigating Escherichia coli habitat transition from sediments to water in tropical urban lakes

Abstract

Background Escherichia coli is a commonly used faecal indicator bacterium to assess the level of faecal contamination in aquatic habitats. However, extensive studies have reported that sediment acts as a natural reservoir of E. coli in the extraintestinal environment. E. coli can be released from the sediment, and this may lead to overestimating the level of faecal contamination during water quality surveillance. Thus, we aimed to investigate the effects of E. coli habitat transition from sediment to water on its abundance in the water column. Methods This study enumerated the abundance of E. coli in the water and sediment at five urban lakes in the Kuala Lumpur-Petaling Jaya area, state of Selangor, Malaysia. We developed a novel method for measuring habitat transition rate of sediment E. coli to the water column, and evaluated the effects of habitat transition on E. coli abundance in the water column after accounting for its decay in the water column. Results The abundance of E. coli in the sediment ranged from below detection to 12,000 cfu g–1, and was about one order higher than in the water column (1 to 2,300 cfu mL–1). The habitat transition rates ranged from 0.03 to 0.41 h–1. In contrast, the E. coli decay rates ranged from 0.02 to 0.16 h−1. In most cases (>80%), the habitat transition rates were higher than the decay rates in our study. Discussion Our study provided a possible explanation for the persistence of E. coli in tropical lakes. To the best of our knowledge, this is the first quantitative study on habitat transition of E. coli from sediments to water column.

Keywords

• Escherichia coli
• Faecal indicator bacteria
• Sediment
• Decay rate
• Habitat transition