Agriculture (Dec 2023)

Genome Survey of <i>Stipa breviflora</i> Griseb. Using Next-Generation Sequencing

  • Xiangjun Yun,
  • Jinrui Wu,
  • Bo Xu,
  • Shijie Lv,
  • Le Zhang,
  • Wenguang Zhang,
  • Shixian Sun,
  • Guixiang Liu,
  • Yazhou Zu,
  • Bin Liu

DOI
https://doi.org/10.3390/agriculture13122243
Journal volume & issue
Vol. 13, no. 12
p. 2243

Abstract

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Due to climate change and global warming, the frequency of sandstorms in northern China is increasing. Stipa breviflora, a dominant species in Eurasian grasslands, can help prevent desertification from becoming more serious. Studies on S. breviflora cover a wide range of fields. To the best of our knowledge, the present study is the first to sequence, assemble, and annotate the S. breviflora genome. In total, 2,781,544 contigs were assembled, and 2,600,873 scaffolds were obtained, resulting in a total length of 649,849,683 bp. The number of scaffolds greater than 1 kb was 70,770. We annotated the assembled genome (>121 kb), conducted a selective sweep analysis, and ultimately succeeded in assembling the Matk gene of S. breviflora. More importantly, our research identified 26 scaffolds that may be responsible for the drought tolerance of S. breviflora Griseb. In summary, the data obtained regarding S. breviflora will be of great significance for future research.

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