Nature Communications (Feb 2021)
Positioning of nucleosomes containing γ-H2AX precedes active DNA demethylation and transcription initiation
- Stephanie Dobersch,
- Karla Rubio,
- Indrabahadur Singh,
- Stefan Günther,
- Johannes Graumann,
- Julio Cordero,
- Rafael Castillo-Negrete,
- Minh Bao Huynh,
- Aditi Mehta,
- Peter Braubach,
- Hector Cabrera-Fuentes,
- Jürgen Bernhagen,
- Cho-Ming Chao,
- Saverio Bellusci,
- Andreas Günther,
- Klaus T. Preissner,
- Sita Kugel,
- Gergana Dobreva,
- Malgorzata Wygrecka,
- Thomas Braun,
- Dulce Papy-Garcia,
- Guillermo Barreto
Affiliations
- Stephanie Dobersch
- Univ Paris Est Creteil, Glycobiology, Cell Growth and Tissue Repair Research Unit (Gly-CRRET), Brain and Lung Epigenetics (BLUE)
- Karla Rubio
- Univ Paris Est Creteil, Glycobiology, Cell Growth and Tissue Repair Research Unit (Gly-CRRET), Brain and Lung Epigenetics (BLUE)
- Indrabahadur Singh
- Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research
- Stefan Günther
- ECCPS Bioinformatics and Deep Sequencing Platform
- Johannes Graumann
- Biomolecular Mass Spectrometry, Max-Planck-Institute for Heart and Lung Research
- Julio Cordero
- Anatomy and Developmental Biology, CBTM
- Rafael Castillo-Negrete
- Univ Paris Est Creteil, Glycobiology, Cell Growth and Tissue Repair Research Unit (Gly-CRRET), Brain and Lung Epigenetics (BLUE)
- Minh Bao Huynh
- Univ Paris Est Creteil, Glycobiology, Cell Growth and Tissue Repair Research Unit (Gly-CRRET), F-
- Aditi Mehta
- Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research
- Peter Braubach
- Institute for Pathology, Hannover Medical School
- Hector Cabrera-Fuentes
- Faculty of Medicine, Department of Biochemistry, Justus Liebig University
- Jürgen Bernhagen
- Chair of Vascular Biology, Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University (LMU) Munich
- Cho-Ming Chao
- Cardio-Pulmonary Institute
- Saverio Bellusci
- Cardio-Pulmonary Institute
- Andreas Günther
- Member of the Universities of Giessen and Marburg Lung Center (UGMLC)
- Klaus T. Preissner
- Faculty of Medicine, Department of Biochemistry, Justus Liebig University
- Sita Kugel
- Human Biology Division, Fred Hutchinson Cancer Research Center
- Gergana Dobreva
- Anatomy and Developmental Biology, CBTM
- Malgorzata Wygrecka
- Member of the Universities of Giessen and Marburg Lung Center (UGMLC)
- Thomas Braun
- Department of Cardiac Development, Max-Planck-Institute for Heart and Lung Research
- Dulce Papy-Garcia
- Univ Paris Est Creteil, Glycobiology, Cell Growth and Tissue Repair Research Unit (Gly-CRRET), F-
- Guillermo Barreto
- Univ Paris Est Creteil, Glycobiology, Cell Growth and Tissue Repair Research Unit (Gly-CRRET), Brain and Lung Epigenetics (BLUE)
- DOI
- https://doi.org/10.1038/s41467-021-21227-y
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 20
Abstract
The order of DNA methylation and histone modifications during transcription remained unclear. Here the authors show that HMGA2 induces DNA nicks at TGFB1-responsive genes, promoting nucleosome incorporation containing γ-H2AX, which is required for repair-mediated DNA demethylation and transcription.
