A brief study about the preservation of Echinococcus granulosus protoscolices in vitro for a long periods.

Abstract

Simple attempt were applied to keep the protoscolices of Echinococcus granulosus removed from human hydatid cysts alive with full activity in vitro using different culture media [RPMI-1640, Physiological Ringer’s solution(RS), Physiological normal saline solution(NS)], the best temperature for cultivation in this media were also investigated between three degrees (4, 25, 37)°C . The best medium for cultivation with a high activity was (NS) solution where the protoscolices continue for (75) days in (25)°C. Whereas the best temperature for cultivation was variable according to the cultivation medium.