Effects of Agrobacterium sp. I26, Manure and Inorganic Fertilizers to Pb Content of Rice Grains Planted in Pb Polluted Soil

Abstract

Chemical waste from textile industries discharged directly into rivers will affect paddy soil irrigation surrounding these factories. Thus, heavy metal pollution may occur in this paddy soil. Bioremediation can remediate polluted heavy metals by removing the pollutant. Agrobacterium sp. I26 and manure were studied as a bioremediation agent because both use biological processes in remediation. The effectivity of bioremediation agent (Agrobacterium sp. I26 or manure) and inorganic fertilizer in inhibiting the absorption of Lead (Pb) in rice, as well as the production of rice, was studied. This study used a factorial Randomized Completely Block Design (RCBD), which consisted of two factors: a) inorganic fertilizers (P): without inorganic fertilizers (P0) and with inorganic fertilizers (P1); b) bioremediation agents (K): without bioremediation agents (K0), with Agrobacterium sp. I26 (K1), with manures (K2). From these two factors, six treatment combinations with four repetitions, resulting in 24 experiment units were obtained. Results of this study showed that Agrobacterium sp. I26 and manures are able to inhibit Pb absorption in rice grains. The best treatment of this study was the combination of inorganic fertilizers with Agrobacterium sp. I26, which showed the highest weight of 1000 seeds (31.95 g), 14.96% higher compared to control, and was able to inhibit Pb absorption by rice grain up to a threshold (0.29 μg/g), 39.58% lower compared with control.

Keywords

• bioremediation
• lead
• agrobacterium sp. i26
• manure
• soil