The survival of Lactobacilli and Agrobacteria introduced into plant phyllosphere

Abstract

The survival of bacteria Lactobacillus plantarum ОNU 12 on leaf surfaces of Kalanchoe daigremontiana Mill., green shoots of grape Vitis vinifera L. cultivar Pinot noire, stems of tomato plants Lycopersicon esculentum Mill. cultivar Ballada, and the joint survival of L. plantarum ОNU 12 and the phytopathogenic bacteria Rhizobium radiobacter C58 in case of the simultaneous inoculation of kalanchoe leaf surfaces was investigated. It was shown that the lactic acid bacteria survived on tomato and grape stems for 30 days and on kalanchoe leaves more than 30 days. In any variant of the simultaneous inoculations with lactic acid bacteria, R. radiobacter C58 was not found on the surfaces of plants already after the third day of the experiment. In case of the simultaneous application with the phytopathogen, lactic acid bacteria L. plantarum ONU 12 survived on plant surfaces for 8 days whereas being inoculated before or after the phytopathogen – survived for 14 days. The protective activity of lactic acid bacteria in inhi­biting the crown gall on test plants was shown. Lactic acid bacteria L. plantarum ONU 12 appeared to be the perspective microorganisms for biological protection against infection caused by the phytopathogen Rhizobium radiobacter and R. vitis.

Keywords

• Lactobacillus plantarum
• Rhizobium radiobacter
• introduction
• plants
• survival