Relationship Between Expression of MicroRNA-150 and Tumor-associated Macrophages in Oral Squamous Cell Carcinoma

Abstract

Objective To investigate the expression of miRNA-150 and IL-10 in TAMs and its clinicopathological significance in OSCC. Methods Different expression of miRNA in OSCC and control was screened by gene chip. The expression of miRNA-150 and CD68/IL-10 protein in oral tissues from 45 cases of OSCC and 38 cases of normal oral mucosa were detected by qRT-PCR and dual immunofluorescence staining. The relationship between miRNA-150 expression and clinical data was analyzed. Results The expression of miRNA-150 and CD68/IL-10 in OSCC tissues was significantly higher than in normal tissues (P < 0.05). The infiltration of CD68+IL-10+ cells increased, and miRNA-150 was related to pathological staging, tumor differentiation and lymph node metastasis in patients with OSCC (P < 0.05). Conclusion The expression of miRNA-150 and TAM-derived IL-10 in OSCC is increased, suggesting that miRNA-150 may promote phenotype transformation of M2 macrophages participating in immunosuppressive function and oral tumorigenesis.

Keywords

• oral squamous cell carcinoma
• il-10
• tumor-associated macrophages