Analyzing gut microbiota composition in individual Anopheles mosquitoes after experimental treatment
Aminata Fofana,
Mathilde Gendrin,
Ottavia Romoli,
G. Armel Bienvenu Yarbanga,
Georges Anicet Ouédraogo,
Rakiswende Serge Yerbanga,
Jean-Bosco Ouédraogo
Affiliations
Aminata Fofana
Institut de Recherche en Sciences de la Santé, Bobo Dioulasso, Burkina Faso; Université Nazi Boni, Bobo-Dioulasso 1091, Burkina Faso
Mathilde Gendrin
Microbiota of Insect Vectors Group, Institut Pasteur de la Guyane, 97306 Cayenne, French Guiana; Institut Pasteur, Université de Paris, Department of Insect Vectors, 75015 Paris, France; Corresponding author
Ottavia Romoli
Microbiota of Insect Vectors Group, Institut Pasteur de la Guyane, 97306 Cayenne, French Guiana
G. Armel Bienvenu Yarbanga
Institut de Recherche en Sciences de la Santé, Bobo Dioulasso, Burkina Faso
Georges Anicet Ouédraogo
Université Nazi Boni, Bobo-Dioulasso 1091, Burkina Faso
Rakiswende Serge Yerbanga
Institut de Recherche en Sciences de la Santé, Bobo Dioulasso, Burkina Faso; Institut des Sciences et Techniques, 2779 Bobo Dioulasso, Burkina Faso
Jean-Bosco Ouédraogo
Institut de Recherche en Sciences de la Santé, Bobo Dioulasso, Burkina Faso; Institut des Sciences et Techniques, 2779 Bobo Dioulasso, Burkina Faso
Summary: The microbiota of Anopheles mosquitoes influences malaria transmission. Antibiotics ingested during a blood meal impact the mosquito microbiome and malaria transmission, with substantial differences between drugs. Here, we assessed if amoxicillin affects the gut mosquito microbiota. We collected Anopheles larvae in Burkina Faso, kept them in semi-field conditions, and offered a blood meal to adult females. We tested the impact of blood supplementation with two alternative amoxicillin preparations on microbiota composition, determined by high-throughput sequencing in individual gut samples. Our analysis detected four major genera, Elizabethkingia, Wigglesworthia, Asaia, and Serratia. The antibiotic treatment significantly affected overall microbiota composition, with a specific decrease in the relative abundance of Elizabethkingia and Asaia during blood digestion. Besides its interest on the influence of amoxicillin on the mosquito microbiota, our study proposes a thorough approach to report negative-control data of high-throughput sequencing studies on samples with a reduced microbial load.
