An enzymatic method for the determination of the initial rate of cholesterol esterification in human plasma

Abstract

A method is described for the determination of the initial rate of cholesterol esterification in human plasma, based on the enzymatic determination of free cholesterol in the plasma before and after incubation at 37°C. The cholesterol esterification rate was linear up to 40 minutes. In 18 normal male and 10 normal female subjects the cholesterol esterification rate was 91 ± 15 (mean ± SD) and 62 ± 12 nmoles/hr/ml of plasma, respectively.

Keywords

• cholesterol esterification
• LCAT
• unesterified and total cholesterol