Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism

Fang Jiao; François Dehez; Tao Ni; Xiulian Yu; Jeremy S. Dittman; Robert Gilbert; Christophe Chipot; Simon Scheuring

doi:10.1038/s41467-022-32757-4

Nature Communications (Aug 2022)

Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism

Fang Jiao,
François Dehez,
Tao Ni,
Xiulian Yu,
Jeremy S. Dittman,
Robert Gilbert,
Christophe Chipot,
Simon Scheuring

Affiliations

Fang Jiao: Department of Anesthesiology, Weill Cornell Medicine
François Dehez: Laboratoire International Associé, Centre National de la Recherche Scientifique et University of Illinois at Urbana-Champaign, Unité Mixte de Recherche no 7019, Université de Lorraine
Tao Ni: Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive
Xiulian Yu: Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive
Jeremy S. Dittman: Department of Biochemistry, Weill Cornell Medicine
Robert Gilbert: Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Roosevelt Drive
Christophe Chipot: Laboratoire International Associé, Centre National de la Recherche Scientifique et University of Illinois at Urbana-Champaign, Unité Mixte de Recherche no 7019, Université de Lorraine
Simon Scheuring: Department of Anesthesiology, Weill Cornell Medicine

DOI: https://doi.org/10.1038/s41467-022-32757-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

Read online

Direct visualization of the structural transformation from pre-pore to pore of the mammalian immune defense complex perforin-2 (PFN2) reveals a clockwise hand-over-hand mechanism that propagates at ~15 subunits per second.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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