The EV antibody database: An interactive database of curated antibodies for extracellular vesicle and nanoparticle research

Amber Morey; Martin Ng; Michail Spanos; Piyan Zhang; Tuoye Xu; Willi Cheung; Emeli Chatterjee; Priyanka Gokulnath; Natacha Carnel-Amar; Ana Luisa Soares Chiaretti; Collin Nelson; Jubin George; Michelle Luo; Abhik Chakraborty; Luiza Perucci; Jennifer C. Jones; Peter De Hoff; Jeffrey L. Franklin; Robert L. Raffai; Saumya Das; David A. Routenberg; John P. Nolan; Al Charest; Louise C. Laurent; Roger P. Alexander

Extracellular Vesicle (Jun 2024)

The EV antibody database: An interactive database of curated antibodies for extracellular vesicle and nanoparticle research

Amber Morey,
Martin Ng,
Michail Spanos,
Piyan Zhang,
Tuoye Xu,
Willi Cheung,
Emeli Chatterjee,
Priyanka Gokulnath,
Natacha Carnel-Amar,
Ana Luisa Soares Chiaretti,
Collin Nelson,
Jubin George,
Michelle Luo,
Abhik Chakraborty,
Luiza Perucci,
Jennifer C. Jones,
Peter De Hoff,
Jeffrey L. Franklin,
Robert L. Raffai,
Saumya Das,
David A. Routenberg,
John P. Nolan,
Al Charest,
Louise C. Laurent,
Roger P. Alexander

Affiliations

Amber Morey: University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA
Martin Ng: Department of Veterans Affairs, Surgical Service (112G), San Francisco VA Medical Center, San Francisco, CA 94121, USA
Michail Spanos: Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Piyan Zhang: Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA
Tuoye Xu: Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA
Willi Cheung: University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA
Emeli Chatterjee: Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Priyanka Gokulnath: Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Natacha Carnel-Amar: Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Ana Luisa Soares Chiaretti: Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Collin Nelson: Meso Scale Diagnostics, Rockville, MD, 20850, USA
Jubin George: Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA
Michelle Luo: Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA
Abhik Chakraborty: University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA
Luiza Perucci: University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA
Jennifer C. Jones: Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA
Peter De Hoff: University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA
Jeffrey L. Franklin: Department of Medicine, Vanderbilt University Medical Center, Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37232, USA
Robert L. Raffai: Department of Veterans Affairs, Surgical Service (112G), San Francisco VA Medical Center, San Francisco, CA 94121, USA; Department of Surgery, Division of Endovascular and Vascular Surgery, University of California, San Francisco, CA 94143, USA
Saumya Das: Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
David A. Routenberg: Meso Scale Diagnostics, Rockville, MD, 20850, USA
John P. Nolan: Scintillon Institute, San Diego, CA, 92121, USA; Cellarcus Biosciences, La Jolla, CA, 92037, USA
Al Charest: Beth Israel Deaconess Medical Center, Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA; Corresponding author.
Louise C. Laurent: University of California San Diego, Department of Obstetrics, Gynecology, and Reproductive Medicine, La Jolla, CA 92093, USA; Corresponding author.
Roger P. Alexander: Extracellular RNA Communication Consortium, Phoenix, AZ 85254, USA; Corresponding author.

Journal volume & issue: Vol. 3
p. 100040

Abstract

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Antibodies are critical tools for research into extracellular vesicles (EVs) and other extracellular nanoparticles (ENPs), where they can be used for their identification, characterization, and isolation. However, the lack of a centralized antibody platform where researchers can share validation results thus minimizing wasted personnel time and reagents, has been a significant obstacle. Moreover, because the performance of antibodies varies among assay types and conditions, detailed information on assay variables and protocols is also of value. To facilitate sharing of results on antibodies that are relevant to EV/ENP research, the EV Antibody Database has been developed by the investigators of the Extracellular RNA Communication Consortium (ERCC). Hosted by the ExRNA Portal (https://exrna.org/resources/evabdb/), this interactive database aggregates and shares results from antibodies that have been tested by research groups in the EV/ENP field. Currently, the EV Antibody Database includes modules for antibodies tested for western Blot, EV Flow Cytometry, and EV Sandwich Assays, and holds 110 records contributed by 6 laboratories from the ERCC. Detailed information on antibody sources, assay conditions, and results is provided, including negative results. We encourage ongoing expert input and community feedback to enhance the database's utility, making it a valuable resource for comprehensive validation data on antibodies and protocols in EV biology.

Published in Extracellular Vesicle

ISSN: 2773-0417 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Biology (General); Medicine: Medicine (General)
Website: https://www.sciencedirect.com/journal/extracellular-vesicle

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