Evalutation of Antimicrobial Effect of Ozonated Water for Disinfection Dental Impressions

Abstract

Introduction: Presence of microbes on the surface of dental impressions poses a risk of spread of infection on dental office staff and also on dental technicians. The standard disinfection of dental impressions is carried out by immersion in disinfective solution or spraying by disinfectant. The commonly used solutions are based on aldehydes, quarter ammonium, isopropyl alcohol, kalium-peroxomonosulphate, tartar acid and chlorine. Regular interchange of disinfective agents is required because of microorganisms' resistance. We consider ozone, which possesses antimicrobial, antiviral and antimycotic properties, as a promising disinfective agent. Aim: The aim of our study was to evaluate efficacy of ozonated water as a possible disinfective agent for dental impressions. Materials and methods: A total amount of 180 impressions were taken from 90 volunteers. First group of 90 impressions served as control group and second group was treated by antimicrobial ozonated water with concentration of 8, 10, 12, 13.5 mg/l and exposition time of 10, 15 and 20 minutes. Samples were cultivated on hard and fluid growth media. Bacteriologic examination of aerobic, microaerobic and anaerobic bacteria was carried out. Results: Solution of 13.5 mg of ozone in 1 liter of water eliminated all microorganisms in all groups of samples. Conclusion: 13.5 mg/l concentration of ozone in water is thought to be a promising agent for disinfection of dental impressions.

Keywords

• ozonated water
• disinfection of dental impressions
• antimicrobial effect