Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1

Katsiaryna Tarasava; Silke Johannsen; Eva Freisinger

doi:10.3390/molecules181114414

Molecules (Nov 2013)

Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1

Katsiaryna Tarasava,
Silke Johannsen,
Eva Freisinger

Affiliations

Katsiaryna Tarasava: Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland
Silke Johannsen: Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland
Eva Freisinger: Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland

DOI: https://doi.org/10.3390/molecules181114414
Journal volume & issue: Vol. 18, no. 11
pp. 14414 – 14429

Abstract

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The first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic g-Ec-1 domain of the wheat MT Ec-1 retains its ability to coordinate two Zn(II) or Cd(II) ions and adopts a three-dimensional structure that is highly similar to the one of the linear wild-type form. However, the reduced flexibility of the protein backbone facilitates structure solution significantly and results in a certain stabilization of metal binding to the protein.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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