Nature Communications (Feb 2020)

De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

  • Limin Shi,
  • Chenxu Yan,
  • Zhiqian Guo,
  • Weijie Chi,
  • Jingle Wei,
  • Weimin Liu,
  • Xiaogang Liu,
  • He Tian,
  • Wei-Hong Zhu

DOI
https://doi.org/10.1038/s41467-020-14615-3
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantification.