School of Biomedical Sciences,, The University of Hong Kong, Hong Kong; The University of Hong Kong Shenzhen of Research Institute and Innovation (HKU-SIRI), Shenzhen, China
Centre for Blood Research, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
Mateusz Kudelko
School of Biomedical Sciences,, The University of Hong Kong, Hong Kong
Rakesh Sharma
Proteomics and Metabolomics Core Facility, The University of Hong Kong, Hong Kong
Wilson CW Chan
School of Biomedical Sciences,, The University of Hong Kong, Hong Kong; The University of Hong Kong Shenzhen of Research Institute and Innovation (HKU-SIRI), Shenzhen, China; Department of Orthopaedics Surgery and Traumatology, HKU-Shenzhen Hospital, Shenzhen, China
Christopher M Overall
Centre for Blood Research, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
School of Biomedical Sciences,, The University of Hong Kong, Hong Kong; The University of Hong Kong Shenzhen of Research Institute and Innovation (HKU-SIRI), Shenzhen, China
The spatiotemporal proteome of the intervertebral disc (IVD) underpins its integrity and function. We present DIPPER, a deep and comprehensive IVD proteomic resource comprising 94 genome-wide profiles from 17 individuals. To begin with, protein modules defining key directional trends spanning the lateral and anteroposterior axes were derived from high-resolution spatial proteomes of intact young cadaveric lumbar IVDs. They revealed novel region-specific profiles of regulatory activities and displayed potential paths of deconstruction in the level- and location-matched aged cadaveric discs. Machine learning methods predicted a ‘hydration matrisome’ that connects extracellular matrix with MRI intensity. Importantly, the static proteome used as point-references can be integrated with dynamic proteome (SILAC/degradome) and transcriptome data from multiple clinical samples, enhancing robustness and clinical relevance. The data, findings, and methodology, available on a web interface (http://www.sbms.hku.hk/dclab/DIPPER/), will be valuable references in the field of IVD biology and proteomic analytics.
