Association of ANRIL Expression with Coronary Artery Disease in Type 2 Diabetic Patients

Abstract

Objective ANRIL is an important antisense noncoding RNA gene in the INK4 locus (9p21.3), a hot spot region associated with multiple disorders including coronary artery disease (CAD), type 2 diabetes mellitus (T2DM) and many different types of cancer. It has been shown that its expression is dysregulated in a variety of immune-mediated diseases. CAD is a major problem in T2DM patients and the cause of almost 60% of deaths in these patients worldwide. The aim of the present study was to compare the expression level of ANRIL between T2DM patients with and without CAD. Materials and Methods In this case-control study, we examined ANRIL expression in peripheral blood mononuclear cell samples by quantitative reverse transcriptionpolymerase chain reaction (RT-qPCR) in 64 T2DM patients with and without CAD (33 CAD+ and 31 CADpatients respectively, established by coronary angiography). Results Expression analysis revealed that ANRIL was up regulated (2.34-Fold, P=0.012) in CAD+ versus CAD diabetic patients. Data from receiver operating characteristic (ROC) curve analysis has shown that ANRIL could act as a potential biomarker for detecting CAD in diabetic patients. Conclusion The expression level of ANRIL is associated with presence of CAD in diabetic patients and could be considered as a potential peripheral biomarker.

Keywords

• ANRIL
• Coronary Artery Disease
• Gene Expression
• Noncoding RNA
• Type 2 Diabetes Mellitus