Preparation and evaluation of the immune efficacy of an inactivated fowl adenovirus 8a serotype oil emulsion vaccine

Jingqi Wu; Xiao Lu; Lingling Song; Liping Liu; Yuehua Gao; Hongmei Li; Kexiang Yu; Lihong Qi

Heliyon (Mar 2024)

Preparation and evaluation of the immune efficacy of an inactivated fowl adenovirus 8a serotype oil emulsion vaccine

Jingqi Wu,
Xiao Lu,
Lingling Song,
Liping Liu,
Yuehua Gao,
Hongmei Li,
Kexiang Yu,
Lihong Qi

Affiliations

Jingqi Wu: Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, 271018, China; College of Veterinary Medicine, Shandong Agricultural University, Tai’an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, 271018, China
Xiao Lu: Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan, China
Lingling Song: Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan, China
Liping Liu: Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan, China
Yuehua Gao: Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan, China
Hongmei Li: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, 271018, China; College of Veterinary Medicine, Shandong Agricultural University, Tai’an, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai’an, 271018, China; Corresponding author. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai’an, 271018, China
Kexiang Yu: Poultry Institute, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan, China; Corresponding author.
Lihong Qi: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 5
p. e26578

Abstract

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In recent years, fowl adenovirus (FAdV) transmission has significantly increased worldwide, leading to substantial economic losses in the poultry industry. The virus causes hepatitis-hydropericardium syndrome (HHS) and inclusion body hepatitis (IBH). The prevalent FAdV strains in China are FAdV-4, FAdV-8a, FAdV-8b, and FAdV-11. Vaccines for FAdV-4 and FAdV-8b, which prevent HHS and IBH, are available commercially, but no vaccine exists for FAdV-8a. To address this issue, we developed a vaccine using an oil emulsion to inactivate the FAdV-8a serotype. Additionally, we built a fluorescence quantitative PCR for the detection of the virus. The lowest concentration detected was 4.11 × 101 copies/μL. The study's results illustrated that the FAdV-8a oil emulsion vaccine effectively produced significant antibodies and offered ample protection for poultry. This vaccine can potentially limit the transmission of IBH resulting from FAdV-8a in China.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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