Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu, Seoul 02841, Korea
Sung Eun Kim
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 08308, Korea
Young-Pil Yun
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 08308, Korea
Somang Choi
Department of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu, Seoul 02841, Korea
Hak-Jun Kim
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 08308, Korea
Kyeongsoon Park
Department of Systems Biotechnology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, Korea
Hae-Ryong Song
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80, Guro-dong, Guro-gu, Seoul 08308, Korea
The purpose of this study was to evaluate the potential of porous poly (d,l-lactic-co-glycolic acid) (PLGA) microspheres (PMSs) immobilized on biphasic calcium phosphate nanoparticles (BCP NPs) (BCP-IM-PMSs) to enhance osteogenic activity. PMSs were fabricated using a fluidic device, and their surfaces were modified with l-lysine (aminated-PMSs), whereas the BCP NPs were modified with heparin–dopamine (Hep-DOPA) to obtain heparinized–BCP (Hep-BCP) NPs. BCP-IM-PMSs were fabricated via electrostatic interactions between the Hep-BCP NPs and aminated-PMSs. The fabricated BCP-IM-PMSs showed an interconnected pore structure. In vitro studies showed that MG-63 cells cultured on BCP-IM-PMSs had increased alkaline phosphatase activity, calcium content, and mRNA expression of osteocalcin (OCN) and osteopontin (OPN) compared with cells cultured on PMSs. These data suggest that BCP NP-immobilized PMSs have the potential to enhance osteogenic activity.
