Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women

Abstract

Objective:Prior studies have shown that receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) have an essential role in bone remodeling and osteoporosis. This study aimed to investigate the association between RANKL (TNFSF11) and OPG (TNFRSF11B) genes’ polymorphisms with low bone mineral density (BMD) in Indonesian postmenopausal women.Materials and Methods:Sixty postmenopausal women aged between 50-65 years were eligible. The BMD was measured by using dualenergy X-ray absorptiometry. The genotypes of TNFSF11 and TNFRSF11B were obtained by polymerase chain reaction-restriction fragment length polymorphisms and DNA sequencing methods. Three single nucleotide polymorphisms (SNPs) from TNFSF11 (-290C>T, -643C>T, -693G>C) and from TNFRSF11B (163A>G, 950T>C, 1181G>C) were selected. The association between alleles distribution and BMD was computed using chi-square or Fischer’s exact test. Multiple logistic regression was used to analyze between all SNPs and BMD at bone sites. Significance was set at p0.05). The distribution of genotypes and alleles in TNSSF11 and TNFRSF11B between healthy and low BMD were not significantly different (all p>0.05). There was no association between SNPs of TNFSF11 and TNFRSF11B with BMD at all bone sites (all p>0.05).Conclusion:The present study suggests that TNFSF11 and TNFRSF11B gene polymorphisms are not associated with BMD in Indonesian postmenopausal women aged between 50-65 years old.

Keywords

• Bone mineral density
• RANKL gene
• OPG gene
• polymorphism
• postmenopause