Frontiers in Microbiology (Dec 2014)

Nucleotide Sequence of Conjugative Prophage Φ1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for efflux resistance to macrolides in Streptococcus pyogenes

  • Francesco eIannelli,
  • Maria eSantagati,
  • Francesco eSantoro,
  • Marco R Oggioni,
  • Stefania eStefani,
  • Gianni ePozzi

DOI
https://doi.org/10.3389/fmicb.2014.00687
Journal volume & issue
Vol. 5

Abstract

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Genetic element Φ1207.3 (formerly Tn1207.3) is a prophage of Streptococcus pyogenes which carries the macrolide efflux resistance genes mef(A)/msr(D) and is capable of conjugal transfer among streptococci. Complete nucleotide sequence showed that Φ1207.3 is 52,491 bp in length and contained 58 ORFs. A manual homology-based annotation with functional prediction of the hypothetical gene product was possible only for 34 out of 58 ORFs. Φ1207.3 codes for two different C-methylation systems, several phage structural genes, a lysis cassette (composed by a holin and a peptidoglycan hydrolase), and three site-specific resolvases of the serine recombinase family.

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