Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

Sukanya Chumchuen; Chonthicha Satirapod; Amornrat Tangprasittipap; Suradej Hongeng

Stem Cell Research (Apr 2022)

Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

Sukanya Chumchuen,
Chonthicha Satirapod,
Amornrat Tangprasittipap,
Suradej Hongeng

Affiliations

Sukanya Chumchuen: Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
Chonthicha Satirapod: Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Amornrat Tangprasittipap: Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand; Corresponding author.
Suradej Hongeng: Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand

Journal volume & issue: Vol. 60
p. 102675

Abstract

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MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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