Yeast Synthetic Biology for the Production of <i>Lycium barbarum</i> Polysaccharides
Jinjin Peng,
Luan Wang,
Mengge Wang,
Rui Du,
Shangshang Qin,
Cheng-Yun Jin,
Yongjun Wei
Affiliations
Jinjin Peng
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
Luan Wang
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
Mengge Wang
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
Rui Du
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
Shangshang Qin
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
Cheng-Yun Jin
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
Yongjun Wei
Key Laboratory of Advanced Drug Preparation Technologies, School of Pharmaceutical Sciences, Zhengzhou University, Ministry of Education, Zhengzhou 450001, Henan, China
The fruit of Lycium barbarum L. (goji berry) is used as traditional Chinese medicine, and has the functions of immune regulation, anti-tumor, neuroprotection, anti-diabetes, and anti-fatigue. One of the main bioactive components is L. barbarum polysaccharide (LBP). Nowadays, LBP is widely used in the health market, and it is extracted from the fruit of L. barbarum. The planting of L. barbarum needs large amounts of fields, and it takes one year to harvest the goji berry. The efficiency of natural LBP production is low, and the LBP quality is not the same at different places. Goji berry-derived LBP cannot satisfy the growing market demands. Engineered Saccharomyces cerevisiae has been used for the biosynthesis of some plant natural products. Recovery of LBP biosynthetic pathway in L. barbarum and expression of them in engineered S. cerevisiae might lead to the yeast LBP production. However, information on LBP biosynthetic pathways and the related key enzymes of L. barbarum is still limited. In this review, we summarized current studies about LBP biosynthetic pathway and proposed the strategies to recover key enzymes for LBP biosynthesis. Moreover, the potential application of synthetic biology strategies to produce LBP using engineered S. cerevisiae was discussed.
