Postnatal hyperosmolality alters development of hypothalamic feeding circuits with context-specific changes in ingestive behavior
Serena R. Sweet,
Jessica E. Biddinger,
Jessie B. Zimmermann,
Gina L. Yu,
Richard B. Simerly
Affiliations
Serena R. Sweet
Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Center for Addiction Research, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37232, USA
Jessica E. Biddinger
Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Center for Addiction Research, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37232, USA
Jessie B. Zimmermann
Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA
Gina L. Yu
Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA
Richard B. Simerly
Department of Molecular Physiology & Biophysics, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Center for Addiction Research, Vanderbilt University, Nashville, TN 37232, USA; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37232, USA; Corresponding author
Summary: Drinking and feeding are tightly coordinated homeostatic events and the paraventricular nucleus of the hypothalamus (PVH) represents a possible node of neural integration for signals related to energy and fluid homeostasis. We used TRAP2;Ai14 mice and Fos labeling to visualize neurons in the PVH and median preoptic nucleus (MEPO) responding to both water deprivation and feeding signals. We determined that structural and functional development of dehydration-sensitive inputs to the PVH precedes those of agouti-related peptide (AgRP) neurons, which convey hunger signals and are known to be developmentally programmed by nutrition. Moreover, we found that osmotic hyperstimulation of neonatal mice led to enhanced AgRP inputs to the PVH in adulthood, as well as disruptions to ingestive behaviors during high-fat diet feeding and dehydration-anorexia. Thus, development of feeding circuits is impacted not only by nutritional signals, but also by early perturbations to fluid homeostasis with context-specific consequences for coordination of ingestive behavior.
