Nature Communications (Feb 2020)
Haploid genetic screens identify SPRING/C12ORF49 as a determinant of SREBP signaling and cholesterol metabolism
- Anke Loregger,
- Matthijs Raaben,
- Joppe Nieuwenhuis,
- Josephine M. E. Tan,
- Lucas T. Jae,
- Lisa G. van den Hengel,
- Sebastian Hendrix,
- Marlene van den Berg,
- Saskia Scheij,
- Ji-Ying Song,
- Ivo J. Huijbers,
- Lona J. Kroese,
- Roelof Ottenhoff,
- Michel van Weeghel,
- Bart van de Sluis,
- Thijn Brummelkamp,
- Noam Zelcer
Affiliations
- Anke Loregger
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- Matthijs Raaben
- Oncode Institute, Division of Biochemistry, The Netherlands Cancer Institute
- Joppe Nieuwenhuis
- Oncode Institute, Division of Biochemistry, The Netherlands Cancer Institute
- Josephine M. E. Tan
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- Lucas T. Jae
- Oncode Institute, Division of Biochemistry, The Netherlands Cancer Institute
- Lisa G. van den Hengel
- Oncode Institute, Division of Biochemistry, The Netherlands Cancer Institute
- Sebastian Hendrix
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- Marlene van den Berg
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- Saskia Scheij
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- Ji-Ying Song
- Division of Experimental Animal Pathology, The Netherlands Cancer Institute
- Ivo J. Huijbers
- Mouse Clinic for Cancer and Aging (MCCA) Transgenic Facility, The Netherlands Cancer Institute
- Lona J. Kroese
- Mouse Clinic for Cancer and Aging (MCCA) Transgenic Facility, The Netherlands Cancer Institute
- Roelof Ottenhoff
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- Michel van Weeghel
- Laboratory of Genetic and Metabolic Diseases and Core Facility Metabolomics, Academic Medical Center of the University of Amsterdam
- Bart van de Sluis
- Department of Pediatrics, University Medical Center Groningen
- Thijn Brummelkamp
- Oncode Institute, Division of Biochemistry, The Netherlands Cancer Institute
- Noam Zelcer
- Department of Medical Biochemistry, Amsterdam UMC, Amsterdam Cardiovascular Sciences and Gastroenterology and Metabolism, University of Amsterdam
- DOI
- https://doi.org/10.1038/s41467-020-14811-1
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 14
Abstract
The transcription factor SREBP is a well-studied and major regulator of sterol and fatty acid metabolism. Here, the authors used haploid genetic screens to identify the Golgi-resident protein SPRING as a new modulator of SREBP by regulating the level of functional SREBP cleavage-activating protein (SCAP).
