Spontaneous Regeneration of Plantlets Derived from Hairy Root Cultures of <i>Lopezia racemosa</i> and the Cytotoxic Activity of Their Organic Extracts
Norely Vargas-Morales,
Norma Elizabeth Moreno-Anzúrez,
Janeth Téllez-Román,
Irene Perea-Arango,
Susana Valencia-Díaz,
Alfonso Leija-Salas,
Edgar R. Díaz-García,
Pilar Nicasio-Torres,
María Del Carmen Gutiérrez-Villafuerte,
Jaime Tortoriello-García,
Jesús Arellano-García
Affiliations
Norely Vargas-Morales
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico
Norma Elizabeth Moreno-Anzúrez
Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla Km 6, Calle CEPROBI No. 8, Colonia San Isidro C.P., Yautepec 62731, Morelos, Mexico
Janeth Téllez-Román
Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Iztatzíhuatl esq. Leñeros s/n Col. Los Volcanes C.P., Cuernavaca 62350, Morelos, Mexico
Irene Perea-Arango
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico
Susana Valencia-Díaz
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico
Alfonso Leija-Salas
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n Col. Chamilpa C.P., Cuernavaca 62210, Morelos, Mexico
Edgar R. Díaz-García
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico
Pilar Nicasio-Torres
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico
María Del Carmen Gutiérrez-Villafuerte
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico
Jaime Tortoriello-García
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1 Col. Centro C.P., Xochitepec 62790, Morelos, Mexico
Jesús Arellano-García
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa C.P., Cuernavaca 62209, Morelos, Mexico
A histological analysis was performed with the aim of elucidating the spontaneous regeneration process of the hairy root lines LRT 2.3 and LRT 6.4, derived from Lopezia racemosa leaf explants and genetically transformed with the Agrobacterium rhizogenes strain ATCC15834/pTDT. The analysis showed both lines regenerate via indirect somatic embryogenesis; LRT 6.4 also regenerated by direct organogenesis. The morphogenic characteristics of the regenerated plantlets from both lines showed the typical characteristics, described previously, including a higher number of axillary shoot formation, short internodes, and plagiotropic roots compared with wild-type seedlings. The regeneration process occurred without the addition of plant growth regulators and was linked to the sucrose concentration in the culture medium. Reducing the sucrose concentration from 3% to 2%, 1%, and 0.5% increased the regeneration rate in LRT 6.4; the effect was less pronounced in LRT 2.3. The cytotoxic activity of different organic extracts obtained from roots and shoots were evaluated in the cancer cell lines HeLa (cervical carcinoma), HCT-15 (colon adenocarcinoma), and OVCAR (ovary carcinoma). The hexane and dichloromethane extracts from roots of both lines showed cytotoxic activity against the HeLa cell line. Only the dichloromethane extract from the roots of PLRT 2.3 showed cytotoxic activity against the OVCAR cell line. None of the methanol extracts showed cytotoxic activity, nor the shoot extracts from any solvent.
