Interaction of Copper, Ascorbate and Gibberllin on germination, ion content, proline, soluble sugar and starch content two cultivars of rape seed (Brassica napus L.)

Abstract

Copper is one of the heavy toxic metals that its toxicity is currently under investigation, in different plant types considering physiologic, biological, molecular, cellular and genetic aspects. In this research the copper toxicity effect on some of physiological activity of "Brassica napus L." Hylo 401 and RGS has been student. Seed of Brassica were cultured in hydroponic environmental. Roots and shoots used for evaluation of different treatments: Cu (5, 15, 25μm/l), Cu & AsA (0.5μm/l), Cu & GA3 (0.05μm/l) and Cu, AsA & Ga3 (as above), experiments were done in three replications in each treat group. In both Hyola 401 and RGS following factors were decreased significantly (p<0.05); germination percent, unsoluble sugar, K+ ions (in shoot and root). In both Hyola and RGS following factors were increased significantly (P<0.05) soluble sugar, proline. The finding of the study suggest that upon copper stress, in majority of studied parameters, Hyola 401 was more resistant than RGS. AsA treatment accompanying Cu, attenuated Cu effects on Brassica seedling, however GA3 exaggerated the Cu effects. Collected data were exracted and statistical analysis with SPSS statistical software 14th edition, performed. The results suggest that Cu induces inhibitory effects on growth and some of physiologic activities of plnant.

Keywords

• brassica napus l
• copper
• ascorbate
• gibberllin
• stress