SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection
Georgi Dyankov,
Tinko Eftimov,
Evdokiya Hikova,
Hristo Najdenski,
Vesselin Kussovski,
Petia Genova-Kalou,
Vihar Mankov,
Hristo Kisov,
Petar Veselinov,
Sanaz Shoar Ghaffari,
Mila Kovacheva-Slavova,
Borislav Vladimirov,
Nikola Malinowski
Affiliations
Georgi Dyankov
Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, Bulgaria
Tinko Eftimov
Central Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sankt Petersburg Blvd., 4000 Plovdiv, Bulgaria
Evdokiya Hikova
Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, Bulgaria
Hristo Najdenski
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev Str., 1113 Sofia, Bulgaria
Vesselin Kussovski
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev Str., 1113 Sofia, Bulgaria
Petia Genova-Kalou
National Center of Infectious and Parasitic Diseases, 44A “Gen. Stoletov” Blvd., 1233 Sofia, Bulgaria
Vihar Mankov
Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, Bulgaria
Hristo Kisov
Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, Bulgaria
Petar Veselinov
Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, Bulgaria
Sanaz Shoar Ghaffari
Photonics Research Center, Université du Québec en Outaouais, Rue 101 St-Jean Bosco, Gatineau, QC J8X 3G5, Canada
Mila Kovacheva-Slavova
Department of Gastroenterology, University Hospital Tsaritsa Ioanna-ISUL, Medical University Sofia, 8 “Byalo More” Str., 1527 Sofia, Bulgaria
Borislav Vladimirov
Department of Gastroenterology, University Hospital Tsaritsa Ioanna-ISUL, Medical University Sofia, 8 “Byalo More” Str., 1527 Sofia, Bulgaria
Nikola Malinowski
Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, Bulgaria
Given the medical and social significance of Helicobacter pylori infection, timely and reliable diagnosis of the disease is required. The traditional invasive and non-invasive conventional diagnostic techniques have several limitations. Recently, opportunities for new diagnostic methods have appeared based on the recent advance in the study of H. pylori outer membrane proteins and their identified receptors. In the present study we assess the way in which outer membrane protein–cell receptor reactions are applicable in establishing a reliable diagnosis. Herein, as well as in other previous studies of ours, we explore the reliability of the binding reaction between the best characterized H. pylori adhesin BabA and its receptor, the blood antigen Leb. For the purpose we developed surface plasmon resonance (SPR) and double resonance long period grating (DR LPG) biosensors based on the BabA–Leb binding reaction for diagnosing H. pylori infection. In SPR detection, the sensitivity was estimated at 3000 CFU/mL—a much higher sensitivity than that of the RUT test. The DR LPG biosensor proved to be superior in terms of accuracy and sensitivity—concentrations as low as 102 CFU/mL were detected.
