Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder

Abstract

Chicken egg yolk is an easily available source for the isolation of sialylglycopeptides (SGP) carrying homogenous biantennary N-glycans. This approach has gained much attention in the last decade since these SGPs can easily be used for the semi-synthesis of glycoconjugates circumventing laborious full-synthetic methodologies. Here we report an optimised, significantly shorter (one day instead of five) and environmentally friendly procedure for the mg scale isolation of SGP using commercially available egg yolk powder. A single chromatographic step following chloroform/methanol precipitation of proteins and lipids yielded desired approximately 200 mg SGP from 250 g egg yolk powder within a day. • Environmentally friendly procedure for isolation of sialylglycopeptide from Egg yolk powder. • Reduced the protocol from five days down to one. Method name: Improved strategy for large scale isolation and purification of sialylglycopeptide from egg yolk powder, Keywords: Synthetic glycobiology, Glycopeptide isolation, Egg yolk powder