Stable Deuterium Labeling of Histidine-Rich Lysine-Based Dendrimers
Nadezhda N. Sheveleva,
Denis A. Markelov,
Mikhail A. Vovk,
Irina I. Tarasenko,
Mariya E. Mikhailova,
Maxim Yu Ilyash,
Igor M. Neelov,
Erkki Lahderanta
Affiliations
Nadezhda N. Sheveleva
Saint Petersburg State University, 7/9 Universitetskaya nab, 199034 Saint Petersburg, Russia
Denis A. Markelov
Saint Petersburg State University, 7/9 Universitetskaya nab, 199034 Saint Petersburg, Russia
Mikhail A. Vovk
Saint Petersburg State University, 7/9 Universitetskaya nab, 199034 Saint Petersburg, Russia
Irina I. Tarasenko
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi Prospect 31, V.O., 199004 Saint Petersburg, Russia
Mariya E. Mikhailova
Saint Petersburg State University, 7/9 Universitetskaya nab, 199034 Saint Petersburg, Russia
Maxim Yu Ilyash
Saint Petersburg National Research University of Information Technologies, Mechanics and Optics (ITMO University), Kronverkskiy pr. 49, 197101 Saint Petersburg, Russia
Igor M. Neelov
Saint Petersburg National Research University of Information Technologies, Mechanics and Optics (ITMO University), Kronverkskiy pr. 49, 197101 Saint Petersburg, Russia
Erkki Lahderanta
Department of Physics, LUT University, Box 20, 53851 Lappeenranta, Finland
Peptide dendrimers, due to their biocompatibility and low toxicity, are highly promising candidates as nanocarriers for drugs and genes. The development of this kind of delivery system requires reliable monitoring of their metabolic and biological pathways. In this respect, hydrogen isotope labeling has tremendous importance, being a safe tool for detection of the labeled nanocarriers. In this work, we have synthesized new histidine-rich lysine-based dendrimers (Lys-2His dendrimer) with two linear histidine (His) residues in every inner segment. The presence of His residues has enabled us to perform controlled deuteration of Lys-2His dendrimers. The high deuteration degree (around 70%) does not practically change after redissolving the samples in H2O and heating them at 40 °C, which indicates the isotopic label stability.
