Cell Reports
(Aug 2013)
The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells
Göran Karlsson,
Emma Rörby,
Cristina Pina,
Shamit Soneji,
Kristian Reckzeh,
Kenichi Miharada,
Christine Karlsson,
Yanping Guo,
Cristina Fugazza,
Rajeev Gupta,
Joost H.A. Martens,
Hendrik G. Stunnenberg,
Stefan Karlsson,
Tariq Enver
Affiliations
Göran Karlsson
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
Emma Rörby
Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 22184 Lund, Sweden
Cristina Pina
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
Shamit Soneji
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
Kristian Reckzeh
Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 22184 Lund, Sweden
Kenichi Miharada
Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 22184 Lund, Sweden
Christine Karlsson
Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 22184 Lund, Sweden
Yanping Guo
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
Cristina Fugazza
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
Rajeev Gupta
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
Joost H.A. Martens
Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University, 6500HB Nijmegen, the Netherlands
Hendrik G. Stunnenberg
Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University, 6500HB Nijmegen, the Netherlands
Stefan Karlsson
Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 22184 Lund, Sweden
Tariq Enver
Stem Cell Laboratory, University College London Cancer Institute, London WC1E 6BT, UK
DOI
https://doi.org/10.1016/j.celrep.2013.07.020
Journal volume & issue
Vol. 4,
no. 4
Abstract
Read online
Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.
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