Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>

Sebastian Tobias Soukup; Dominic Alexander Stoll; Nicolas Danylec; Alena Schoepf; Sabine Emma Kulling; Melanie Huch

doi:10.3390/foods10112741

Foods (Nov 2021)

Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>

Sebastian Tobias Soukup,
Dominic Alexander Stoll,
Nicolas Danylec,
Alena Schoepf,
Sabine Emma Kulling,
Melanie Huch

Affiliations

Sebastian Tobias Soukup: Department of Safety and Quality of Fruit and Vegetables, Federal Research Institute of Nutrition and Food, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany
Dominic Alexander Stoll: Department of Safety and Quality of Fruit and Vegetables, Federal Research Institute of Nutrition and Food, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany
Nicolas Danylec: Department of Safety and Quality of Fruit and Vegetables, Federal Research Institute of Nutrition and Food, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany
Alena Schoepf: Department of Safety and Quality of Fruit and Vegetables, Federal Research Institute of Nutrition and Food, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany
Sabine Emma Kulling: Department of Safety and Quality of Fruit and Vegetables, Federal Research Institute of Nutrition and Food, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany
Melanie Huch: Department of Safety and Quality of Fruit and Vegetables, Federal Research Institute of Nutrition and Food, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany

DOI: https://doi.org/10.3390/foods10112741
Journal volume & issue: Vol. 10, no. 11
p. 2741

Abstract

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The intake of isoflavones is presumed to be associated with health benefits in humans, but also potential adverse effects of isoflavones are controversially discussed. Isoflavones can be metabolized by gut bacteria leading to modulation of the bioactivity, such as estrogenic effects. Especially bacterial strains of the Eggerthellaceae, a well-known bacterial family of the human gut microbiota, are able to convert the isoflavone daidzein into equol. In addition, metabolization of genistein is also described for strains of the Eggerthellaceae. The aim of this study was to identify and investigate gut bacterial strains of the family Eggerthellaceae as well as the narrowly related family Coriobacteriaceae which are able to metabolize daidzein and genistein. This study provides a comprehensive, polyphasic approach comprising in silico analysis of the equol gene cluster, detection of genes associated with the daidzein, and genistein metabolism via PCR and fermentation of these isoflavones. The in silico search for protein sequences that are associated with daidzein metabolism identified sequences with high similarity values in already well-known equol-producing strains. Furthermore, protein sequences that are presumed to be associated with daidzein and genistein metabolism were detected in the two type strains ‘Hugonella massiliensis’ and Senegalimassilia faecalis which were not yet described to metabolize these isoflavones. An alignment of these protein sequences showed that the equol gene cluster is highly conserved. In addition, PCR amplification supported the presence of genes associated with daidzein and genistein metabolism. Furthermore, the metabolism of daidzein and genistein was investigated in fermentations of pure bacterial cultures under strictly anaerobic conditions and proofed the metabolism of daidzein and genistein by the strains ‘Hugonella massiliensis’ DSM 101782T and Senegalimassilia faecalis KGMB04484T.

Published in Foods

ISSN: 2304-8158 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/foods

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