Strontium and Copper Co-Doped Multifunctional Calcium Phosphates: Biomimetic and Antibacterial Materials for Bone Implants
Vladimir N. Lebedev,
Mariya I. Kharovskaya,
Bogdan I. Lazoryak,
Anastasiya O. Solovieva,
Inna V. Fadeeva,
Abdulkarim A. Amirov,
Maksim A. Koliushenkov,
Farid F. Orudzhev,
Oksana V. Baryshnikova,
Viktoriya G. Yankova,
Julietta V. Rau,
Dina V. Deyneko
Affiliations
Vladimir N. Lebedev
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia
Mariya I. Kharovskaya
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia
Bogdan I. Lazoryak
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia
Anastasiya O. Solovieva
Laboratory of Pharmacology Active Compounds, Research Institute of Clinical and Experimental Lymphology–Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (RICEL–Branch of IC&G SB RAS), 630060 Novosibirsk, Russia
Inna V. Fadeeva
A.A. Baikov Institute of Metallurgy and Material Science RAS, Leninskie, 49, 119334 Moscow, Russia
Abdulkarim A. Amirov
Amirkhanov Institute of Physics, Dagestan Scientific Center of Russian Academy of Sciences, 367003 Makhachkala, Russia
Maksim A. Koliushenkov
Physics Department, Lomonosov Moscow State University, Leninskie Gori 1, 119991 Moscow, Russia
Farid F. Orudzhev
Geothermal and Renewal Energy Institute of the High Temperature Joint Institute of the Russian Academy of Sciences, 367015 Makhachkala, Russia
Oksana V. Baryshnikova
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia
Viktoriya G. Yankova
Institute of Pharmacy, Department of Analytical, Physical and Colloid Chemistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Building 2, 119048 Moscow, Russia
Julietta V. Rau
Institute of Pharmacy, Department of Analytical, Physical and Colloid Chemistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Building 2, 119048 Moscow, Russia
Dina V. Deyneko
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia
β-tricalcium phosphate (β-TCP) is a promising material in regenerative traumatology for the creation of bone implants. Previously, it was established that doping the structure with certain cations can reduce the growth of bacterial activity. Recently, much attention has been paid to co-doped β-TCP, that is explained by their ability, on the one hand, to reduce cytotoxicity for cells of the human organism, on the other hand, to achieve a successful antibacterial effect. Sr, Cu-co-doped solid solutions of the composition Ca9.5–xSrxCu(PO4)7 was obtained by the method of solid-phase reactions. The Rietveld method of structural refinement revealed the presence of Sr2+ ions in four crystal sites: M1, M2, M3, and M4. The M5 site is completely occupied by Cu2+. Isomorphic substitution of Ca2+ → (Sr2+and Cu2+) expands the concentration limits of the existence of the solid solution with the β-TCP structure. No additional phases were formed up to x = 4.5 in Ca9.5–xSrxCu(PO4)7. Biocompatibility tests were performed on cell lines of human bone marrow mesenchymal stromal cells (hMSC), human fibroblasts (MRC-5) and osteoblasts (U-2OS). It was demonstrated that cytotoxicity exhibited a concentration dependence, along with an increase in osteogenesis and cell proliferation. Ca9.5–xSrxCu(PO4)7 powders showed significant inhibitory activity against pathogenic strains Escherichia coli and Staphylococcus aureus. Piezoelectric properties of Ca9.5–xSrxCu(PO4)7 were investigated. Possible ways to achieve high piezoelectric response are discussed. The combination of bioactive properties of Ca9.5–xSrxCu(PO4)7 renders them multifunctional materials suitable for bone substitutes.
