Extension professionals were, and in many cases still are, heavily involved in assessing how the COVID-19 pandemic impacted agribusiness operations and the broader food system. This publication describes the process that a core project team of Extension professionals and researchers from the University of Florida and Florida Gulf Coast University followed to design and implement an interview guide to harmonize the collection of such information from food system businesses in Florida. We discuss benefits of this method, tools that improved efficiency during implementation, and considerations for others who might want to modify and use this interview guide in their own state or region.