Generic Variance Indicating Mutations in The Vp2 Gene of Infectious Bursal Disease Virus (Ibdv) Variants

Abstract

Infectious bursal disease (IBD) is a disease caused by infectious bursa virus (IBDV). The disease is highly affecting young chickens, representing a serious threat to the poultry industry worldwide. This research aims to determine the pattern of mutation and the trend of evolutionary relationship among different variants of infectious bursal disease virus (IBDV). A total of 26 sequences of VP2 gene of different IBDV variants, was retrieved from the National Centre of Biotechnology Information (NCBI BLAST) Gen Bank. Multiple sequence alignment was carried out with AF248612.1 as reference sequence. The nucleotide sequences were also translated to amino acids and checked the mutation based on the amino acids’ substitutions. From the result, mutation was observed at position S-254-G, E-270-A and S-335-V. However, phylogenetic study categorized the IBDV variants into different geographical continents which comprises Malaysia, China, Finland, Iran, Brazil, Korean, Algeria, Japan, Israel, Russia Germany, and UK Isolates. Understanding the mutation particularly in the VP2 gene may provide insight on the pattern of amino acid substitution which may ease the selection of variant for best vaccine candidate.

Keywords

• VP2 Gene
• Amino acids
• Mutation
• Phylogenetic