Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes

Renee G.C. Maas; Soah Lee; Magdalena Harakalova; Christian J.B. Snijders Blok; William R. Goodyer; Jesper Hjortnaes; Pieter A.F.M. Doevendans; Linda W. Van Laake; Jolanda van der Velden; Folkert W. Asselbergs; Joseph C. Wu; Joost P.G. Sluijter; Sean M. Wu; Jan W. Buikema

STAR Protocols (Mar 2021)

Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes

Renee G.C. Maas,
Soah Lee,
Magdalena Harakalova,
Christian J.B. Snijders Blok,
William R. Goodyer,
Jesper Hjortnaes,
Pieter A.F.M. Doevendans,
Linda W. Van Laake,
Jolanda van der Velden,
Folkert W. Asselbergs,
Joseph C. Wu,
Joost P.G. Sluijter,
Sean M. Wu,
Jan W. Buikema

Affiliations

Renee G.C. Maas: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands
Soah Lee: Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
Magdalena Harakalova: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands
Christian J.B. Snijders Blok: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands
William R. Goodyer: Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
Jesper Hjortnaes: Department of Cardiothoracic Surgery, Heart & Lung Center, Leiden University Medical Center, Leiden, the Netherlands
Pieter A.F.M. Doevendans: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands
Linda W. Van Laake: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands
Jolanda van der Velden: Amsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Centers, Amsterdam, the Netherlands
Folkert W. Asselbergs: Department of Cardiology, Division Heart & Lungs, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Institute of Cardiovascular Science, Faculty of Population Health Sciences, University College London, London, UK; Health Data Research UK and Institute of Health Informatics, University College London, London, UK
Joseph C. Wu: Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA
Joost P.G. Sluijter: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands
Sean M. Wu: Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Corresponding author
Jan W. Buikema: Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 3508 GA Utrecht, the Netherlands; Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100334

Abstract

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Summary: Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modeling, drug and toxicity screening, and cell therapy.For complete details on the use and execution of this protocol, please refer to Buikema (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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