Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs)
Mickaël Ménager,
Dan Littman
Affiliations
Mickaël Ménager
The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, USA
Dan Littman
The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, USAHoward Hughes Medical Institute, Washington, USA
The aim of this protocol is to describe how to measure and quantify the amount of HIV-1 particles and dextran molecules internalized in human monocyte derived dendritic cells (MDDCs), using three different techniques: flow cytometry, quantitative PCR and confocal microscopy.
