Medicinal plant as herbal medicine is widely used to cure infectious diseases. Rubus fruticosus L (R. fruticosus) has been studied for its antimicrobial and cytotoxic activities. Different polarity leave extracts were prepared by using hexane, chloroform, ethyl acetate and hydro alcoholic solvents. Agar diffusion method has been used to assess the antibacterial activity against two gram-positive Enterococcus faecalis and Staphylococcus aureus and two gram-negative Escherichia coli and Haemophilus influenza bacterial strains. Cytotoxic activity was carried out against brine shrimp using the nauplii method (BSL). The results so obtained for various experiments demonstrates the total number of extracts produces moderate to strong antibacterial activity against the gram (+ and -). The best activity was discovered within hydro alcoholic upon all concentrations, whereas the inhibition zone exists within the range of 6−11 mm. In addition to that, the cytotoxic activity test confirms that hydro alcoholic extracts the maximum toxicity for values LC50 (4.68–6.96 μg/ml), where the LC50 values for all extracts be located within the range of 4.68–6.96 μg/ml. Finally, the plant itself and its derived extracts have been used as a folk medicine to treat serious infectious diseases.