Chemical mutagenesis in head cabbage (Brassica oleracea var. capitata L.): IV DNA polymorphism

Abstract

ISSR method was applied for a detection of induced variability on a DNA level in M1 generation after Ethylmethan Sulphonate (EMS) chemical treatment in concentrations 0.5, 0.6 and 0.7% of seeds from head cabbage, Brassica oleraceae L, var. Capitata, cv. Ditmarsko. Different patterns of mutant plants obtained from the studied species were established by randomly selected primer sequences among tandem repeats. Polymorphisms were revealed amongM1 and control plants. Diverse DNA patterns of mutant plants were identified. As a result of the pre-breeding screening done in M1, altered plants were selected and seeds f or growing the next M2 generation were gathered.

Keywords

• head cabbage
• Brassica oleraceae L.
• ISSR-PCR
• induced mutagenesis
• EMS
• DNA polymorphism